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| 1. | 3' (downstream) end and 5' (upstream) end, respectively of the transcription unit |
| 2. | 5' (upstream) end and 3' (downstream) end, respectively of the transcription unit |
| 3. | the 5' (upstream) end |
| 4. | the 3' (downstream) end |

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| 1. | exons and introns do not appear in the mature RNA |
| 2. | exons appear but introns do not appear in the mature RNA |
| 3. | introns appear but exons do not appear in the mature RNA |
| 4. | both exons and introns appear in the mature RNA |

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